KnowItAll Solutions for GC-MS
Accelerate your spectral analyses & streamline your workflow!
KnowItAll offers solutions to identify, analyze, and manage your GC-MS data. Combined with Wiley’s comprehensive, high-quality spectral reference databases, it provides one of the most advanced technologies available for fast, reliable spectral analysis.
Find out what's new in KnowItAll 2025
Automate unknown identification
Go from raw chromatogram to identifications with a single click
Leverage high-quality KnowItAll libraries*
Identify faster with access to up to millions of spectra across techniques
Flexible, vendor neutral for easy integration
Supports multiple formats & techniques: future proofs your lab as you add or change instruments
Key features
| Explore the benefits of KnowItAll GC-MS solutions |
|---|
| Automated non-targeted GC-MS analysis |
| Spectral search |
| Processing |
| High-quality GC-MS database collections* |
| Database building & data management (Supports multiple techniques & instruments) |
| Integrated toolsets for structure drawing, reporting, & more |
| Quantitation tool |
 | Comprehensive, integrated tools for efficient analysis. By combining all the tools and data you need into a single, easy-to-use interface, KnowItAll will save you time, improve workflow, and increase your ability to reach conclusions from your data. |
 | Vendor neutral for seamless integration. Supports multiple file & instrument formats, so even if you change or add instruments, your workflow remains intact. |
 | VPAT compliant. With accessibility features like keyboard access to menus, audio narration for icons, and tool tips. |
 | Multi-language interface. Software is currently available in English, Chinese, French, German, and Japanese. |
*Subscription required to KnowItAll Spectral Libraries.
Automated non-targeted GC-MS analysis
MS Expert – Combines deconvolution with spectral search into an automated workflow
GC-MS data analysis can be time consuming, especially when examining complex analytes. MS Expert automatically processes, deconvolutes, and analyzes GC-MS data.
Combined with KnowItAll’s fast database search, it accurately suggests matches to knowns and allows unknowns to be further examined.
How Does It Work?
Simply import a GC-MS data file and the software automatically deconvolutes the TIC into components. All extracted component GC-MS spectra are then automatically searched against reference libraries* to find matches. Results are shown as a hit list for each component.
Unidentified components or components with low match scores can then be sent to KnowItAll’s SearchIt tool for manual investigation. In SearchIt, one can use adaptive search (patented) to match similar components or use mixture analysis to separate coeluted components.
Spectral Search
Sophisticated GC-MS search for confident identification & classification
Wiley offers sophisticated tools to search and compare unknown mass spectra with KnowItAll’s high-quality reference spectra* or user generated databases.
With fast search speeds, powerful algorithms, and advanced technologies, Wiley aims to deliver results you can rely on.
Additional search tools
NEW! Wiley’s patented adaptive search for MS:
This technology finds spectral matches that are similar to the unknown but have additional or missing selective fragment(s). It then suggests what might be causing the differences, where possible. This feature provides tremendous insight into structural possibilities to explore when there is no exact match. Ultimately, this may lead to more intelligent and confident identification and confirmation.
Mixture Analysis of MS Spectra:
One of the most powerful features is KnowItAll’s ability to analyze mixtures. When searching an unknown against a reference database, you can choose to search for multiple components. The result is a series of composite spectra, each accompanied by the individual component spectra that comprise the composite spectrum, as well as the residual spectrum (difference between the query and the composite spectrum). Composite spectra are then ranked by how closely they resemble the query spectrum.
Processing
GC-MS Data Processing
To empower researchers, KnowItAll 2025 revamps manual GC-MS processing to provide users greater control of component separation and identification. Manual processing features were made available directly in MS Expert. Users can view and select a mass spectrum or the average of mass spectra and define the background. The selected mass spectrum or extracted spectra can be transferred for database searching or imported into a user database. These improvements, combined with the completely automated data analysis in the previous release, make KnowItAll 2025 an even more reliable, efficient, powerful, and accurate tool for GC-MS data analysis.
High-quality GC-MS database collections
The Most Comprehensive Data for Results You Can Rely On
Spectral analysis software is not complete without a high-quality database of reference spectra. And with KnowItAll, Wiley offers the best of both!
The KnowItAll GC-MS Library* is a trusted data source that provides over 956,000 mass spectra of the highest quality.
Increase access to over 1.3 million spectra by adding NIST and other MS subscription options!
Spectral Data Management
Build Single or Multi-Technique Databases from Various Vendors
Because Wiley Science Solution’s primary business is spectral databases, KnowItAll is built through years of experience in doing just that—building databases.
Researchers can build searchable databases that include one or more analytical techniques (IR, MS, Near IR, NMR, Raman, UV-Vis), chemical structures, and other meta data. So even if a laboratory’s instruments come from multiple manufacturers, KnowItAll can archive the data.
Integrated tools for structure drawing, reporting, & more
ChemWindow 2D structure drawing & reporting tools
Because structures are critical to your work, we’ve integrated ChemWindow, a trusted software used by chemists worldwide, into KnowItAll to streamline your workflow. This integration allows you to draw and use structures directly within KnowItAll, eliminating the need to switch between programs.
And with KnowItAll’s integrated ReportIt tool, you can effortlessly generate reports, papers, and presentations to showcase your spectral identification results, complete with spectra, structures, annotations, data tables, and more.
And Many More Value-Added MS Tools!
Mass Fragmentation Tool
Isotopic Distribution Tool
Elemental Composition Calculator
Quantitation Tool
Consolidate your software and analytical workflow using KnowItAll’s integrated quantitation tool**
Wiley’s KnowItAll has always helped us answer the question of “what” is the identify of a substance. KnowItAll helps answer the question of “how much” of the substance there is with an integrated quantitation tool* to complete and simplify your analytical workflow.
Because all the tools and techniques are integrated into a single environment, KnowItAll makes the laboratory workflow seamless.
** This valuable quantitation tool is now included with KnowItAll Analytical Edition software and KnowItAll Campus-Wide subscriptions.
Applications
This versatile solution is useful for accelerating workflows across a range of applications, such as:
- Pharmaceuticals
- Drug Design & metabolomics
- Extractables & leachables
- Environmental monitoring
- Forensics
- Food & beverage
- Cosmetics
- Biotechnology
- Materials science
- Academic research & teaching
- Many more
Compatibility
KnowItAll’s vendor-neutral environment supports multiple instrument formats across a range of techniques, allowing for easy integration. This flexibility helps your workflow remain intact as you add or change instruments, ensuring seamless operation and continuous efficiency.
KnowItAll and ChemWindow are trademarks of John Wiley & Sons, Inc. in certain jurisdictions.
*Subscription required to KnowItAll Spectral Libraries.
 | Discover how KnowItAll can revolutionize your GC-MS analysis |