An extract of S. hygroscopicus was separated into 40 fractions using high-performance liquid chromatography (HPLC). The fractions were tested for growth inhibition of B. burgdorferi, resulting in the isolation of a pure compound with a molecular mass of 511 Da (Figure 1A). The structure was determined by a combination of mass spectrometry (MS) and NMR analysis (Figures S1 and S2; Tables S1 and S2). Surprisingly, a search of AntiBase database revealed the identity of this compound to hygromycin A, a known antibiotic (Figure 1A).

View Case Study