What's New in KnowItAll 2025
KnowItAll is better than ever with new tools and advances for spectral analysis.
Wiley’s KnowItAll spectral software offers solutions to identify, analyze, and manage analytical and chemical data. Combined with Wiley’s comprehensive, high-quality spectral reference libraries, it provides an unparalleled solution for fast, reliable spectral analysis.
KnowItAll 2025, the latest version of Wiley’s comprehensive software suite for spectral analysis and analytical data management, introduces a groundbreaking new tool to automate LC-MS analysis, enhanced data management, and numerous other features to streamline analysis across multiple spectral techniques.
Below is a summary of the many exciting updates. Access to features and data depends on the user’s license and edition of the software you are using.
New options you can add to your KnowItAll workflows*
NEW! KnowItAll LC Expert: Streamline component identification with the power of the new KnowItAll LC Expert’s automated workflow.
Simply open your LC-MS chromatogram in LC Expert to deconvolute it into extracted ion chromatograms (EICs). The correlated MS/MS spectra are then automatically matched against Wiley’s LC-MS databases* to identify them. LC Expert features both MS/MS and accurate mass search methods for the most complete analysis.
Users will have access to more search algorithms in KnowItAll’s SearchIt application. This includes the renowned MSforID search algorithm. Also new in KnowItAll 2025 for LC-MS: Patent-Pending Adaptive Search is available for both low-resolution and high-resolution instruments.
High-quality LC-MS/MS database collections
Spectral analysis software is not complete without high-quality databases of reference spectra. As a leader in spectral data, Wiley offers the best of both with this latest release. Users can access up to 5M+ tandem MS spectra depending on the databases they license, including the new KnowItAll LC-MS Library collection and the following databases now available in KnowItAll format: Maurer, Meyer, Helfer, Weber: LC-HR-MS/MS Library of Drugs, Poisons, and Their Metabolites, Maurer, Wissenbach, Weber: LC-MSn Library of Drugs, Poisons, and Their Metabolites, 2nd Edition, or NIST MS/MS Library.
NEW! KnowItAll Data Control: Securely manage and share analytical databases across your organization
KnowItAll Data Control seamlessly integrates KnowItAll’s powerful spectral data management tools with your organization’s file sharing server to add a layer of security to your shared databases. It provides a secure and efficient way to manage your valuable analytical databases, ensuring they are accessible to those who need it, while maintaining control over changes to it.
NEW! KnowItAll drug classification models
Designed to help forensics laboratories classify novel compounds, as drugs continue to be synthesized faster than reference data can be produced. IR, GC-MS, and Raman models currently available.
Additional highlights in the 2025 release
- Process It application:
- New Raman processing algorithms: Fluorescence corrections and cosmic ray removal now available
- LC-MS and GC-MS processing are now rolled into the respective MS Expert applications (ProcessIt application is now for IR, Raman, NMR processing only)
- Can now add peak labels (This data is transferable to MineIt and ReportIt)
- MS Expert application:
- Streamlined GC-MS workflow: Automatic deconvolution, peak picking, and manual analysis are now combined into one unified application
- Improved component chromatogram display: allows editing, table information lookup using CTRL+F searching.
- Streamlined GC-MS workflow: Automatic deconvolution, peak picking, and manual analysis are now combined into one unified application
- Quantitation application:
- Now included with the KnowItAll Analytical Edition
- Allows the user to force fit a line through the origin (0,0)
- Added weighting to regression
- User can enter data in table format
- NMR tools expanded to support XNMR: New tools introduced in KnowItAll 2024 for CNMR and HNMR are now available for XNMR data.
- View query spectrum metadata: Users can now see the metadata for their imported query spectrum in SearchIt, ID Expert, AnalyzeIt, and ReportIt.
- ID Expert application:
- Can toggle on/off cosmic ray detection and removal
- “Classification” tab appears showing spectrum classification results for any classification models you have subscribed to
- SearchIt application:
- Users can now open a spectrum directly from the Peak Search (no longer need to import from Spectrum Search)
- Accurate mass search has been added
- MineIt application:
- Simplified export menu and export options
- Added support for XRD and XRF
- Users can now send feedback on data direct to our product team from MineIt
- ChemWindow application:
- New way to add pre-defined substituents (“Insert Predefined Substituent” menu item added)
- OPSIN Name2Structure function has been improved
- Elemental Composition Calculator: Now has ring double bond equivalency (RDBE) calculation
- Training Documents Updated: Our extensive KnowItAll tutorial documents have been updated and new chapters have been added. This series of lessons provides tutorials the user can follow along with here
Compatibility
KnowItAll and ChemWindow are trademarks of John Wiley & Sons, Inc. in certain jurisdictions.
*Subscription required to spectral libraries and various features/options.